E.coli

In order to examine bacterial transformation, an experiment was conducted in which E.coli bacteria were treated with calcium chloride and heat shock. They were then were given a plasmid containing genes for ampicillin-resistance and pFluoroGreen™. Bacteria were then grown on ampicillin containing agar to select the bacteria with the plasmid. This experiment was conducted to show that E.coli could be transformed using plasmid and that the bacteria can be selected out when placed in an antibiotic solution. After the experiment, the bacteria were observed to see if the pFluoroGreen™ was produced by using long-wave ultraviolet light to see if the bacteria fluoresced. Several colonies grew on the ampicillin containing agar plate, and they fluoresced under ultraviolet light, which showed successful transformation of the E.coli. If the E.coli are given a plasmid and a proper heat shock the E.coli will transform and produce a desired protein

- This was my Abstract from my research paper in which I wrote for a Science-Mathematics-Technology honors program at my school.